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BAC libraries available at CNRGV

The CNRGV in France has more than 3 million BAC clones from wheat BAC libraries available for the scientific community.

http://cnrgv.toulouse.inra.fr/en/library/wheat

The French Plant Genomic Resources Centre (CNRGV), unique in France, was established in 2004 as a nonprofit Biological Resource Center (BRC) dedicated to plant genomic resources. The activities of this national organisation are to ensure that this important genomic material and their descriptions are maintained and available for the benefit of science and biotechnology. Since its creation, CNRGV has centralized more than 7 millions clones derived from genomic resources of model and crop plants: Arabidopsis thaliana, Medicago truncatula, wheat, tomato, pepper, grapevine, rapeseed, maize, barley, sunflower, tobacco, radish, melon, etc. Among them, more than 3 million BAC clones are from wheat BAC libraries (libraries described in the table below and http://cnrgv.toulouse.inra.fr/en/library/wheat). The objectives of the CNRGV are also to produce efficient tools for genome analysis and make them available for the scientific community. In this context, the CNRGV has developed rapid, efficient, and cheap tools to exploit BAC libraries in order to isolate positive clones located in regions of interest. For example, three-dimensional pools coupled with PCR screening are used in various projects for isolation of genes of interest, construction of physical map of a region, identification of homoeologous genes in polyploid species, and comparisons of genomic studies between crop and model species, More information is available at http://cnrgv.toulouse.inra.fr/en

Table 1: Description of the wheat BAC libraries available at CNRGV

 

BAC Library Name Restriction Enzyme Mean Insert size Genome equivalent
TaaCsp3BFhA HindIII 103 kb 6,2 X
TaaCsp3BFhB HindIII 126 kb 10,5 x
TaaCsp3BFhA_MTP HindIII 103 kb 1 x
TaaCsp3DLhA HindIII 105 kb 11 x
TaaCsp3DLhA_MTP HindIII 105 kb 1 x
TaaCsp3DShA HindIII 110 kb 11 x
TaaCsp3DShA_MTP HindIII 110 kb 1 x
TaaCsp1ALhA HindIII 103kb 8 X
TaaCsp1ALhA _MTP HindIII 103kb 1 X
TaaCsp1ALhB HindIII 109kb 7,7 X
TaaCsp1ALhB _MTP HindIII 109kb 1 X
TaaCsp1AL _MTP HindIII 109kb 1 X
TaaCsp1AShA HindIII 111kb 11,8x
TaaCsp1AShA _MTP HindIII 111kb 1x
TaaCsp1BLhA HindIII 114kb 15,4x
TaaCsp1BLhA_MTP HindIII 114kb 1x
TaaCsp1BShA HindIII 115kb 15,7x
TaaCsp1BShA_MTP HindIII 115kb 1x
TaaCsp5ASha HindIII 120 kb 16,5x
Tae-B-CS, Chinese spring HindIII 130 kb 9,3 X
Tae-B-Renan (3 sub-libraries) HindIII-EcoRI-BamHI 110 kb 6, 9 X
Ttu-B-LDN65 (Triticum turgidum) HindIII 130 kb 5 X