Home / Projects / IWGSC Bread Wheat Projects / Sequencing / Whole Chromosome Reference Sequencing Projects / 3BSEQ

Sequencing chromosome 3B

Targeted chromosomes

3B
3B
Click on a chromosome to access associated data (when available) at URGI Sequences Repository.

Project team

Project Leader: Frederic Choulet (frederic.chouletSPAMFILTER@clermont.inra.fr)
First nameLast nameEmailInstitutionCountry
Catherine FEUILLET INRA France
Etienne PAUX etienne.pauxSPAMFILTER@clermont.inra.fr INRA France
Philippe LEROY philippe.leroySPAMFILTER@clermont.inra.fr INRA France
Pierre SOURDILLE pierre. sourdilleSPAMFILTER@clermont.inra.fr INRA France
Nicolas GUIHLOT nicolas.guihlotSPAMFILTER@clermont.inra.fr INRA France

Project collaborators

First nameLast nameEmailInstitutionCountry
Patrick WINCKER pwinckerSPAMFILTER@genoscope.cns.fr Genoscope France
Hadi QUESNEVILLE Hadi.QuesnevilleSPAMFILTER@versailles.inra.fr INRA URGI France
Jaroslav DOLEZEL dolezelSPAMFILTER@ueb.cas.cz IEB Czech Republic
Hélène BERGES hbergesSPAMFILTER@toulouse.inra.fr INRA CNRGV France
Michael ALAUX michael.alauxSPAMFILTER@versailles.inra.fr INRA-URGI France

Project funding

Funding was awarded by the French Research Agency ANR and France Agrime r to the group led by Catherine Feuillet (Structure, function and evolution of the wheat genomes) at INRA GDEC Clermont Ferrand, France for the complete sequencing of the 1Gb chromosome 3B using a BAC by BAC approach and Next Generation Sequencing technologies. The 3BSEQ project is funded for 3 years (2010-2013) and is developed in partnership with the French National Sequencing center Genoscope and the INRA bioinformatics unit URGI .

Abstract

The objectives of 3BSEQ are to:

  • Sequence and annotate the whole chromosome 3B
  • Understand its evolutionary dynamics through comparative analyses with the other cereal genomes available
  • Functionally characterize the gene space by establishing a transcriptomic map of the chromosome
  • Study intraspecific variations of the 3B gene space (Copy Number and Present-Absent Variations)
  • Develop useful sequence markers for traits of agronomic importance and for molecular breeding

Publications

  • Choulet et al., Structural and Functional Partitioning of Bread Wheat Chromosome 3B, Science , 2014 Jul 18;345(6194):1249721. doi: 10.1126/science.1249721.
  • Glover NM, Daron J, Pingault L, et al. Small-scale gene duplications played a major role in the recent evolution of wheat chromosome 3B. Genome Biology . 2015;16(1):188. doi: 10.1186/s13059-015-0754-6.
  • Pingault, L. et al. Deep transcriptome sequencing provides new insights into the structural and functional organization of the wheat genome. Genome Biol. 16, 29 (2015). doi: 10.1186/s13059-015-0601-9