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Physical mapping of the wheat chromosome 5A

Targeted chromosomes

5A
5A
Click on a chromosome to access associated data (when available) at URGI Sequences Repository.

Project team

First nameLast nameEmailInstitutionCountry
Michele Stanca CRA-Genomic Research Centre Italy
Sergio Lucretti ENEA BIOTEC-GEN Casaccia Research Centre Italy
Delfina Barabaschi CRA-Genomic Research Centre Italy
L Orrù CRA-Genomic Research Centre Italy
E Francia University of Modena & Reggio Emilia Italy
A Fricano Parco Technologico Padano Italy
D Lafiandra University of Tuscia Italy
A Blanco University of Bari Italy
G Vale CRA-Genomic Research Centre Italy

Project collaborators

First nameLast nameEmailInstitutionCountry
Jaroslav Dolezel IEB Czech Republic
Hana Šimková IEB Czech Republic
A Kilian Diversity Arrays Technology Pty Ltd. Australia

Project funding

This project was funded by the Italian Ministry of Agriculture.

Abstract

The project aims to develop a physical map for each arms of the 5A chromosome of the reference cultivar Chinese Spring. The strategy, planed in according with the IWGSC, is based on the purification of single chromosome arm fractions from 5AL and 5AS ditelosomic lines through chromosome sorting. The purified single chromosome arm DNA will be used to produce a BAC library. This work will be carried out in the lab of J Doležel (Czech republic). The BACs will be fingerprinted and the data will yield a Minimum tiling path of BACs and organized in contigs to cover the whole chromosome region.

All marker resources available for Triticeae will be assessed to define an exhaustive set of 5A chromosome markers. Additional markers will be developed through BAC-ends sequencing. Markers will be used for anchoring BAC contigs to the genetic map. The strategy for contig anchoring involve the screening of the 5A BAC libraries that represent the MTP with markers and the use of high-resolution genetic populations for efficient anchoring of genetically closely linked genes/markers. The project will also promotes the use of a next generation sequencing system to develop a first draft of the 5A sequence and a number of activities dedicated to the functional analysis of 5A located genes involved in the determination of quality aspects, biotic and abiotic stress tolerance.