Physical mapping of the wheat chromosome 5A
Targeted chromosomes
|
Project team
| First name | Last name | Institution | Country | |
|---|---|---|---|---|
| Michele | Stanca | CRA-Genomic Research Centre | Italy | |
| Sergio | Lucretti | ENEA BIOTEC-GEN Casaccia Research Centre | Italy | |
| Delfina | Barabaschi | CRA-Genomic Research Centre | Italy | |
| L | Orrù | CRA-Genomic Research Centre | Italy | |
| E | Francia | University of Modena & Reggio Emilia | Italy | |
| A | Fricano | Parco Technologico Padano | Italy | |
| D | Lafiandra | University of Tuscia | Italy | |
| A | Blanco | University of Bari | Italy | |
| G | Vale | CRA-Genomic Research Centre | Italy |
Project collaborators
| First name | Last name | Institution | Country | |
|---|---|---|---|---|
| Jaroslav | Dolezel | IEB | Czech Republic | |
| Hana | Šimková | IEB | Czech Republic | |
| A | Kilian | Diversity Arrays Technology Pty Ltd. | Australia |
Project funding
This project was funded by the Italian Ministry of Agriculture.
Abstract
The project aims to develop a physical map for each arms of the 5A chromosome of the reference cultivar Chinese Spring. The strategy, planed in according with the IWGSC, is based on the purification of single chromosome arm fractions from 5AL and 5AS ditelosomic lines through chromosome sorting. The purified single chromosome arm DNA will be used to produce a BAC library. This work will be carried out in the lab of J Doležel (Czech republic). The BACs will be fingerprinted and the data will yield a Minimum tiling path of BACs and organized in contigs to cover the whole chromosome region.
All marker resources available for Triticeae will be assessed to define an exhaustive set of 5A chromosome markers. Additional markers will be developed through BAC-ends sequencing. Markers will be used for anchoring BAC contigs to the genetic map. The strategy for contig anchoring involve the screening of the 5A BAC libraries that represent the MTP with markers and the use of high-resolution genetic populations for efficient anchoring of genetically closely linked genes/markers. The project will also promotes the use of a next generation sequencing system to develop a first draft of the 5A sequence and a number of activities dedicated to the functional analysis of 5A located genes involved in the determination of quality aspects, biotic and abiotic stress tolerance.