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Physical mapping of the wheat chromosome 5A

Targeted chromosomes

5A
5A
Click on a chromosome to access associated data (when available) at URGI Sequences Repository.

Project team

First nameLast nameEmailInstitutionCountry
Michele Stanca CRA-Genomic Research Centre Italy
Sergio Lucretti ENEA BIOTEC-GEN Casaccia Research Centre Italy
Delfina Barabaschi CRA-Genomic Research Centre Italy
L Orrù CRA-Genomic Research Centre Italy
E Francia University of Modena & Reggio Emilia Italy
A Fricano Parco Technologico Padano Italy
D Lafiandra University of Tuscia Italy
A Blanco University of Bari Italy
G Vale CRA-Genomic Research Centre Italy

Project collaborators

First nameLast nameEmailInstitutionCountry
Jaroslav Dolezel IEB Czech Republic
Hana Šimková IEB Czech Republic
A Kilian Diversity Arrays Technology Pty Ltd. Australia

Project funding

This project was funded by the Italian Ministry of Agriculture.

Abstract

The project to develop a physical map for each of the arms of chromosome 5A from the reference cultivar Chinese Spring is based on BAC libraries developed for each arm by the lab of J. Dolozel (IEB, Czech Republic) (see here or here ). High information content fluorescent (HICF) fingerprints from 36,155 (5AS) and 39,830 (5AL) BACs were assembled with FPC and LTC. Physical maps based on merging the assemblies and contig anchoring to genetic maps contain, respectively in the minimal tile paths identified for sequencing, 325 (5AS) and 618 (5AL) contigs covering over 100% of the estimated lengths of the chromosome arms.

Publications

Physical Mapping of Bread Wheat Chromosome 5A: An Integrated Approach
Delfina Barabaschi, Federica Magni, Andrea Volante, Agata Gadaleta, Hana Šimková, Simone Scalabrin, Maria Lucia Prazzoli, Paolo Bagnaresi, Katia Lacrima, Vania Michelotti, Francesca Desiderio, Luigi Orrù, Valentina Mazzamurro, Agostino Fricano, AnnaMaria Mastrangelo, Paola Tononi, Nicola Vitulo, Irena Jurman, Zeev Frenkel, Federica Cattonaro, Michele Morgante, Antonio Blanco, Jaroslav Doležel, Massimo Delledonne, Antonio M. Stanca, Luigi Cattivelli and Giampiero Valè
doi: 10.3835/plantgenome2015.03.0011

https://dl.sciencesocieties.org/publications/tpg/first-look