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Construction of BAC DNA libraries specific for chromosome 4AL/4AS and positional cloning of gene for adult plant resistance to powdery mildew in wheat

Targeted chromosomes

4A
4A
Click on a chromosome to access associated data (when available) at URGI Sequences Repository.

Project team

First nameLast nameEmailInstitutionCountry
Jaroslav Dolezel dolezelSPAMFILTER@ueb.cas.cz IEB Czech Republic
Kadri Järve kadri.jarveSPAMFILTER@ttu.ee Tallinn University of Technology Estonia
Hana Simkova simkovahSPAMFILTER@ueb.cas.cz IEB Czech Republic
Jan Safar safarSPAMFILTER@ueb.cas.cz IEB Czech Republic
Jan Bartos bartosSPAMFILTER@ueb.cas.cz IEB Czech Republic
Jarmila Cihalikova cihalikovaSPAMFILTER@ueb.cas.cz IEB Czech Republic

Project collaborators

First nameLast nameEmailInstitutionCountry
Pilar Hernandez phernandezSPAMFILTER@ias.csic.es IAS (CSIC) Spain
Ming-Cheng Luo mcluoSPAMFILTER@ucdavis.edu UC, Davis USA
Klaus Mayer K.mayerSPAMFILTER@helmholtz-muenchen.de MIPS Germany

Project funding

This project was funded by the Czech Science Foundation

Abstract

Infection of wheat by fungus B. graminis is causing powdery mildew disease. Recently, non-race-specific powdery mildew resistance gene QPm.tut-4A was mapped on distal end of long arm of wheat chromosome 4A. Here we propose to construct two BAC DNA libraries specific for 4AL/S chromosomal arms. The huge genome and polyploid character hamper positional cloning in wheat. The proposed libraries would become keys for solving the obstruction and stepping stones for creation of physical map of the 4A chromosome. The physical map will facilitate cloning of important genes from the chromosome, including resistance genes to powdery mildew, leaf rust, Hessian fly, yellow rust, stem rust, wheat streak mosaic and Septoria tritici blotch. The main objective of the project is cloning of the powdery mildew resistance gene QPm.tut-4A. High density map of the QPm.tut-4A gene region will be created and BAC contig spanning the region identified. Subsequently, a pooled genomic BAC library will be prepared from the donor of resistance, T. militinae, and will be used to clone the QPm.tut-4A gene