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Construction of BAC DNA libraries specific for chromosome 4AL/4AS and positional cloning of gene for adult plant resistance to powdery mildew in wheat

Targeted chromosomes

Click on a chromosome to access associated data (when available) at URGI Sequences Repository.

Project team

First nameLast nameEmailInstitutionCountry
Jaroslav Dolezel dolezelSPAMFILTER@ueb.cas.cz IEB Czech Republic
Kadri Järve kadri.jarveSPAMFILTER@ttu.ee Tallinn University of Technology Estonia
Hana Simkova simkovahSPAMFILTER@ueb.cas.cz IEB Czech Republic
Jan Safar safarSPAMFILTER@ueb.cas.cz IEB Czech Republic
Jan Bartos bartosSPAMFILTER@ueb.cas.cz IEB Czech Republic
Jarmila Cihalikova cihalikovaSPAMFILTER@ueb.cas.cz IEB Czech Republic

Project collaborators

First nameLast nameEmailInstitutionCountry
Pilar Hernandez phernandezSPAMFILTER@ias.csic.es IAS (CSIC) Spain
Ming-Cheng Luo mcluoSPAMFILTER@ucdavis.edu UC, Davis USA
Klaus Mayer K.mayerSPAMFILTER@helmholtz-muenchen.de MIPS Germany

Project funding

This project was funded by the Czech Science Foundation


Physical maps for 4AS and 4AL have been constructed from chromosome arm specific BAC libraries (see here ). High information content fluorescent (HICF) fingerprints from 32944 (4AS) and 60140 (4AL) BACs have been assembled with LTC to produce physical maps containing 250 and 924 contigs, respectively, covering 86% and 89% of the estimated lengths of the chromosome arms.

Associated Project led by P. Hernandez.

The BAC DNA libraries specific for 4AL/S chromosomal arms and the physical map for chromosome 4A will facilitate cloning of important genes associated with the chromosome, including resistance genes to powdery mildew, leaf rust, Hessian fly, yellow rust, stem rust, wheat streak mosaic and Septoria tritici blotch. The main objective of the project is cloning of the powdery mildew resistance gene QPm.tut-4A. A high density map of the QPm.tut-4A gene region will be created and a BAC contig spanning the region identified. Subsequently, a pooled genomic BAC library will be prepared from a resistant donor, T. militinae, and will be used to clone the QPm.tut-4A gene.