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Construction of BAC DNA libraries specific for chromosome 4AL/4AS and positional cloning of gene for adult plant resistance to powdery mildew in wheat

Targeted chromosomes

4A
4A
Click on a chromosome to access associated data (when available) at URGI Sequences Repository.

Project team

First nameLast nameEmailInstitutionCountry
Jaroslav Dolezel dolezelSPAMFILTER@ueb.cas.cz IEB Czech Republic
Kadri Järve kadri.jarveSPAMFILTER@ttu.ee Tallinn University of Technology Estonia
Hana Simkova simkovahSPAMFILTER@ueb.cas.cz IEB Czech Republic
Jan Safar safarSPAMFILTER@ueb.cas.cz IEB Czech Republic
Jan Bartos bartosSPAMFILTER@ueb.cas.cz IEB Czech Republic
Jarmila Cihalikova cihalikovaSPAMFILTER@ueb.cas.cz IEB Czech Republic

Project collaborators

First nameLast nameEmailInstitutionCountry
Pilar Hernandez phernandezSPAMFILTER@ias.csic.es IAS (CSIC) Spain
Ming-Cheng Luo mcluoSPAMFILTER@ucdavis.edu UC, Davis USA
Klaus Mayer K.mayerSPAMFILTER@helmholtz-muenchen.de MIPS Germany

Project funding

This project was funded by the Czech Science Foundation

Abstract

Physical maps for 4AS and 4AL have been constructed from chromosome arm specific BAC libraries (see CNRGV page ). High information content fluorescent (HICF) fingerprints from 32944 (4AS) and 60140 (4AL) BACs have been assembled with LTC to produce physical maps containing 250 and 924 contigs, respectively, covering 86% and 89% of the estimated lengths of the chromosome arms.

Publications

  • Hernandez, P., Martis, M., Dorado, G., Pfeifer, M., Schaaf, S., Jouve, N., Šimková, H., Valárik, M., Doložel, J., Mayer, K.F. Next generation sequencing and syntenic integration of flow-sorted arms of wheat chromosome 4A exposes the chromosome structure and gene content. Plant J. 2012 69(3):377-86. DOI 10.1111/j.1365-313X.2011.04808.x
  • Randhawa, M., Bansai, U., Valárik, M., Klocová, B., Doložel, J., Bariana, H. Molecular mapping of stripe rust resistance gene Yr51 in chromosome 4AL of wheat. Theor Appl Genet. 2014 127(2):317-24. DOI 10.1007/s00122-013-2220-8
  • Tsõmbalova, J., Karafiátová, M., Vrána, J., Kubaláková, M., Peuša, H., Jakobson, I., Järve, M., Valárik, M., Doložel, J., Järve, K. A haplotype specific to North European wheat (Triticum aestivum L.) Genetic Resources and Crop Evolution; March 2016. DOI 10.1007/s10722-016-0389-9