7B Reference Sequencing
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Norwegian Research Council has funded this project.
The objective of this project is to obtain a high quality sequence of chromosome 7B. In addition, our project aims at resequencing Norwegian cultivars with the aim to develop a SNP chip for marker assisted breeding by Graminor Inc.
The primary research task is to sequence chromosome 7B. In order to achieve this goal, we will construct and anchor to the genetic map, a physical map of the chromosome 7B of Chinese Spring (Research Task 1). This will be done by first constructing BAC libraries for each of the two chromosome arms 7Bs and 7BL, then assemble these into contigs representing the order of DNA segments in the chromosome arms. Bioinformatics tools will be used to structure, relate and comprehensively analyze the large scale genomics data. Databases for data archival, management and dissemination, tools for genomic sequence analysis, and an integrative database for the display of genetic and physical maps linked to markers, traits and phenotypes will be developed based on the existing CIGENE infrastructure. Data will be integrated with the EU-wide TriticeaeGenome bioinformatics platform and other IWGSC bioinformatics efforts and will be linked to established international cereal bioinformatics platforms such as Gramene. These assemblies will then be sequenced to produce a genomic sequence of 7B (Research Task 2). These activities are at the international forefront of plant genomics, and will position Norway as a member of the leading group in plant genome research. In addition to sequencing, the project aims at developing a comprehensive set of molecular markers for Graminors wheat breeding program (Research task 3). As outlined above these markers will be designed based on the resequencing of Norwegian wheat breeding materials based on the genomic sequence from the entire IWGSC project.