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6B Physical Map

Targeted chromosomes

6B
6B
Click on a chromosome to access associated data (when available) at URGI Sequences Repository.

Project team

Project Leader: Hirokazu Handa (hirokazuSPAMFILTER@affrc.go.jp)
First nameLast nameEmailInstitutionCountry
Yasunari Ogihara Kihara Institute for Biological Research Japan
Takeshi Itoh National Institute of Agrobiological Sciences Japan
Takashi Matsumoto National Institute of Agrobiological Sciences Japan
Shuhei Nasuda Kyoto University Japan
Shigeo Takumi takumiSPAMFILTER@kobe-u.ac.jp Kobe University Japan
Toshiki Nakamura tnakaSPAMFILTER@affrc.go.jp Tohoku Agricultural Research Center Japan
Katusuyuki Hayakawa hayakawa.katsuyukiSPAMFILTER@nisshin.com Nisshin Flour Milling Inc. Japan

Project collaborators

First nameLast nameEmailInstitutionCountry
Jaroslav Dolezel IEB Czech Republic
Takashi Endo Kyoto University Japan

Project funding

Project funding for completion of the physical map and for BAC-based sequencing of chromosome 6B has been provided by the Japanese Ministry of Agriculture, Forestry, and Fisheries.

Abstract

With this project, the following activities will be conducted:

  • Construction of BAC libraries and selection of markers unique to chromosome 6B
  • Development of transcriptome resources and their use as markers
  • Construction of BAC-based physical map and sequencing of 6B
  • Development of genome annotation technique for wheat.

Team members include scientists from the National Institute of Agrobiological Sciences, the Kihara Institute for Biological Research in Yokohama City University, Kyoto University, Kobe University, Nisshin Flour Milling Inc., and RIKEN.

Physical maps for 6BS and 6BL have been assembled using FPC from whole genome profile (WGP) sequence tags of 57,600 (6BS) and 76,032 (6BL) BACs from chromosome arm libraries developed in the lab of J. Dolozel (IEB, Czech Republic) (see CNRGV page ). The assembled maps contain 273 and 287 contigs (> 5 BACs), respectively, covering over 100% of the estimated lengths of the chromosome arms.

Publications

A high-resolution physical map integrating an anchored chromosome with the BAC physical maps of wheat chromosome 6B.
Kobayashi, Fuminori; Wu, Jianzhong; Kanamori, Hiroyuki; Tanaka, Tsuyoshi; Katagiri, Satoshi et al. (2015)
BMC genomics vol. 16 (1) p. 595