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Chromosome 3B survey sequence

Targeted chromosomes

3B
3B
Click on a chromosome to access associated data (when available) at URGI Sequences Repository.

Project team

First nameLast nameEmailInstitutionCountry
Frederic Choulet frederic.chouletSPAMFILTER@clermont.inra.fr INRA GDEC France
Etienne Paux etienne.pauxSPAMFILTER@sancy.clermont.inra.fr INRA GDEC France
Philippe Leroy leroySPAMFILTER@sancy.clermont.inra.fr INRA GDEC France
Patrick Wincker pwinckerSPAMFILTER@genoscope.cns.fr Genoscope France
Michael Alaux michael.alauxSPAMFILTER@versailles.inra.fr INRA URGI France
Hadi Quesneville Hadi.QuesnevilleSPAMFILTER@versailles.inra.fr INRA URGI France

Project collaborators

First nameLast nameEmailInstitutionCountry
Jaroslav Dolezel dolezelSPAMFILTER@ueb.cas.cz IEB Olomouc Czech Republic

Project funding

The group "Structure, function and evolution of the wheat genomes" (PI: C. Feuillet) of the INRA GDEC Clermont-Ferrand (France) has been granted by the French Research Agency ANR and France Agrimer to sequence completely the 1 Gb of chromosome 3B using a BAC by BAC approach and Next Generation Sequencing technologies. The 3BSEQ project is funded for 3 years (2010-2013) and is developed in partnership with the French National Sequencing center Genoscope and the INRA bioinformatics unit URGI.

Abstract

In the framework of the 3BSEQ project, a 50x sequence is under production using Illumina GAIIx analyzer (100bp reads) with paired end reads of 600bp on flow-sorted 3B chromosome . The reads will be assembled as well as mapped against the scaffolds resulting from the 454 Titanium pooled BAC sequencing. This will contribute to the IWGSC effort in survey sequencing the 21 bread wheat chromosome and will complete the BAC by BAC sequencing approach by providing additional links to the 454 scaffolds, and possibly permit joining scaffolds from different contigs of the physical map. In addition, the Illumina data will serve to correct errors in the 454 contigs using the procedure described by Aury et al.(BMC Genomics 9, 603 (2008)