7DS physical map

Construction of BAC libraries from 7DS and 7DL has been funded by the Czech Science Foundation. The physical map of 7DS has been funded with internal funding at the Institute of Experimental Biology.

Positional cloning in a huge allopolyploid genome of bread wheat was considered infeasible, mainly due to presence of three homoeologous genomes and large amounts of repetitive sequences. One of strategies enabling to reach this target is based on the use of genomic resources derived from parts of the hexaploid genome. Here we propose construction of two subgenomic BAC libraries specific for short and long arm of chromosome 7D. They will be invaluable resource for creation of physical maps of chromosome 7D and map-based cloning of several agronomically important genes from chromosome 7D of wheat, including genes conferring resistance to green bug, powdery mildew, stem rust, leaf rust, stripe rust, Septoria tritici blotch and Russian wheat aphid. The project will focus on positional cloning of gene Dnx for resistance to Russian wheat aphid (RWA) by creating high-density genetic map for the Dnx region and BAC contig spanning this region. Further, a pooled genomic BAC library will be prepared from a RWA resistant line, which will be used to clone the Dnx gene.

A physical map for 7DS has been assembled using FPC from high information content fluorescent (HICF) fingerprints from 39,765 BACs from the 7DS BAC library (see CNRGV page ). The map contains 698 contigs (> 5 clones) and covers 97% of the estimated length of the chromosome arm.

Staňková, H., Hastie, A.R., Chan, S., Vrána, J., Tulpová, Z., Kubaláková, M., Visendi, P., Hayashi, S., Luo, M., Batley, J., Edwards, D., Doležel, J. and Šimková, H. BioNano genome mapping of individual chromosomes supports physical mapping and sequence assembly in complex plant genomes. Plant Biotechnol. J. (2015), doi: 10.1111/pbi.12513